| Autor: |
Vrublevskaia VV, Musienko VS, Skarga IuIu, Morenkov OS |
| Jazyk: |
ruština |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 2007 May-Jun; Vol. 52 (3), pp. 33-7. |
| Abstrakt: |
A panel of monoclonal antibodies (MAbs) specific to glycoprotein D (gD) of Aujeszky's disease virus (ADV, Suid herpesvirus 1) was produced and characterized. MAbs were used to identify 9 topologically different epitopes and epitopic groups on gD. The majority of the identified epitopes were conformational. Most gD-specific MAbs possessed virus-neutralizing activity in the presence and absence of the complement. MAbs neutralized the virus at the stage of its penetration into the cell and inhibited the cell-to-cell spread of viruses. Two immunodominant epitopes and one immunodominant domain that induce the most prominent humoral immune response were identified when the animals were infected and immunized. A method was developed for affinity purification of ADV glycoprotein D. Immunization of mice with affinity-purified gD induced a strong humoral immune response and protected mice against lethal ADV challenge. In passive immunization, the majority of gD-specific MAbs protected mice against infection. The findings confirm the important role of ADV glycoprotein D in inducing protective anti-ADV immunity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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