| Autor: |
Yakushevska AE; Cellular Architecture and Dynamics, Institute of Biomembranes, Faculty of Science, Padualaan 8, 3584 CH Utrecht, The Netherlands., Lebbink MN, Geerts WJ, Spek L, van Donselaar EG, Jansen KA, Humbel BM, Post JA, Verkleij AJ, Koster AJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of structural biology [J Struct Biol] 2007 Sep; Vol. 159 (3), pp. 381-91. Date of Electronic Publication: 2007 Apr 30. |
| DOI: |
10.1016/j.jsb.2007.04.006 |
| Abstrakt: |
Transmission electron tomography has been used in biological sciences for quite some time and proven to be a valuable tool. However, to date, the different Scanning Transmission modes are almost not used for electron tomography on resin-embedded biological material. We explored different STEM modes on epon-embedded, osmium-uranyl-lead-stained biological material. Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms from the same areas were recorded and compared. Contrast and signal-to-noise ratios were calculated. Template matching was used to validate results obtained in Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms. It is concluded that High Angle Annular Dark Field-STEM gives a five times better contrast and signal-to-noise ratio than Bright Field-TEM. Template matching showed that 1.3 times more information could be extracted from High Angle Annular Dark Field-STEM tomograms than from Bright Field-TEM tomograms. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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