Isolation and characterization of a new 2,4-dinitrophenol-degrading bacterium Burkholderia sp. strain KU-46 and its degradation pathway.

Autor:	Iwaki H; Department of Biotechnology, Faculty of Engineering, Kansai University, Osaka, Japan., Abe K, Hasegawa Y
Jazyk:	angličtina
Zdroj:	FEMS microbiology letters [FEMS Microbiol Lett] 2007 Sep; Vol. 274 (1), pp. 112-7. Date of Electronic Publication: 2007 Jun 21.
DOI:	10.1111/j.1574-6968.2007.00816.x
Abstrakt:	A gram-negative bacterium, strain KU-46, was isolated from agricultural soil contaminated with pesticides and was found to utilize 2,4-dinitrophenol as the sole source of carbon and nitrogen. Based on 16S rRNA gene sequence analysis and its morphological, biochemical, and physiological characteristics, strain KU-46 was identified as a Burkholderia sp. Metabolite analyses by HPLC and liquid chromatography-MS indicated that 4-nitrophenol, 1,4-benzoquinone, and nitrite are the intermediates of 2,4-dinitrophenol metabolism, and 2,4-dinitrophenol is metabolized via 4-nitrophenol to 1,4-benzoquinone by strain KU-46. The 2,4-dinitrophenol degradation pathway enzymes are induced by both 2,4-dinitrophenol and 4-nitrophenol.
Databáze:	MEDLINE
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