[Design of a species-specific DNA probe based on the pFra plasmid of the plague pathogen].

Autor: Shishkina OG, Popov IuA, Kirillina OA, Mashko SV, Lebedeva MI
Jazyk: ruština
Zdroj: Molekuliarnaia genetika, mikrobiologiia i virusologiia [Mol Gen Mikrobiol Virusol] 1991 Oct (10), pp. 16-9.
Abstrakt: The recombinant plasmid pBS1 carrying a 2 kb SalGI fragment of Yersinia pestis pFra plasmid was constructed by insertion of the fragment into a vector plasmid pBR327. SalGI-BspRI 400 bp subfragment was recloned into a pBR322 vector plasmid. Open reading frame was found in the fragment by DNA sequencing technique. The subfragment designated F1-probe permits one to identify specifically the Yersinia pestis strains harbouring pFra plasmid, thus, differing them from closely related Yersiniea and other representatives of Enterobacteriaceae family.
Databáze: MEDLINE