Serial day rapid kindling is an effective tool in screening the anti-epileptic properties of topiramate.

Autor: De Smedt T; Laboratory for Clinical and Experimental Neurophysiology, University Hospital Ghent, Belgium. T.DeSmedt@UGent.be, De Rouck S, Raedt R, Wyckhuys T, Waterschoot L, De Herdt V, Van Dycke A, Tahry RE, Vonck K, Boon P
Jazyk: angličtina
Zdroj: Seizure [Seizure] 2007 Oct; Vol. 16 (7), pp. 620-6. Date of Electronic Publication: 2007 Jun 07.
DOI: 10.1016/j.seizure.2007.04.013
Abstrakt: Introduction: In this study, a serial day rapid kindling protocol was used to fully kindle rats in a matter of days. Subsequently, the anticonvulsant profile of a relatively new anti-epileptic drug, topiramate, was evaluated in a cross-over design to further validate this rapid kindling model.
Methods: Rats were kindled during three consecutive days, according to the serial day rapid kindling protocol. Topiramate was tested at a dose of 100mg/kg, i.p., over the next 2 days using a cross-over design. The stability of the kindled state was evaluated in all rats during two retest paradigms. During the drug-testing procedure, rats received a single i.p. injection of either topiramate or verhicle. Starting 1 h later the rats received additional kindling stimulations during which their response was measured.
Results: Serial day rapid kindling induced a long lasting and stable fully kindled state that allowed for the anti-epileptic drug screening procedure. Topiramate reduced both the afterdischarge duration and ameliorated seizure semiology in the kindled rats.
Discussion: Serial day rapid kindling provided a tool to rapidly kindle rats in 3 days. Using a cross-over design, clear indications on anti-epileptic activity of a given drug can be determined using few laboratory animals.
Databáze: MEDLINE
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