| Autor: |
Sangloung C; Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Buddhirongawatr R, Tantawiwattananon N, Sukthana Y |
| Jazyk: |
angličtina |
| Zdroj: |
The Southeast Asian journal of tropical medicine and public health [Southeast Asian J Trop Med Public Health] 2006; Vol. 37 Suppl 3, pp. 18-20. |
| Abstrakt: |
In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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