Molecular characterisation of kappa- and lambda-carrageenan by gel permeation chromatography, light scattering, sedimentation analysis and osmometry.

Autor: Slootmaekers D; Department of Physical and Macromolecular Chemistry, Gorlaeus Laboratories, University of Leiden, Netherlands., van Dijk JA, Varkevisser FA, van Treslong CJ, Reynaers H
Jazyk: angličtina
Zdroj: Biophysical chemistry [Biophys Chem] 1991 Oct; Vol. 41 (1), pp. 51-9.
DOI: 10.1016/0301-4622(91)87209-n
Abstrakt: Gel permeation chromatography, in conjunction with a double detection system involving a low angle laser light scattering apparatus (LALLS) and a refractive index monitoring device (RI), has been used to obtain both the molecular weight and the molecular weight distribution of sodium salts of kappa-carrageenan and lambda-carrageenan in saline solutions. The results, Mw and Mn, are in excellent agreement with independent determinations of molar mass based on static light scattering experiments, sedimentation-diffusion analysis and osmometry. The relevance of the data is discussed with respect to current problems in carrageenan research.
Databáze: MEDLINE