| Autor: |
Pravin Kumar R; Vittal Mallya Scientific Research Foundation, Bangalore, India., Manoj MN, Kush A, Annadurai RS |
| Jazyk: |
angličtina |
| Zdroj: |
Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2007 Jun; Vol. 37 (6), pp. 635-40. Date of Electronic Publication: 2007 Mar 28. |
| DOI: |
10.1016/j.ibmb.2007.03.010 |
| Abstrakt: |
In silico docking analysis reported here suggests that insect cellular cytoskeletal beta-actin could be the target of Azadirachtin A (Aza-the principle bioactive compound of neem seeds). The best docking energy of -40.09 kcal/mol at 8.73 A RMSD and predicted hydrogen bond between Arg210 and carboxymethyl group of Aza accompanied with seven hydrophobic interactions in the proposed binding site strongly support this hypothesis. This is of specific interest due to the non-affinity of Aza to mammalian beta-actins under the same set of conditions, although beta-actins across the species are highly conserved. Our results show that few scattered amino acid changes have caused significant steric hindrance in the binding pocket for mammalian beta-actin, causing a reverse orientation of Aza. These results suggest a model to support the recently observed biological effects caused by Aza in Drosophila cytoskeletal elements and explain why Aza is highly specific to insects than mammals. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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