| Autor: |
Gil Tejedor AM; Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia, c/Senda del Rey 9, 28040 Madrid, Spain., Fernández Hernando P, Durand Alegría JS |
| Jazyk: |
angličtina |
| Zdroj: |
Analytica chimica acta [Anal Chim Acta] 2007 May 15; Vol. 591 (1), pp. 112-5. Date of Electronic Publication: 2007 Mar 30. |
| DOI: |
10.1016/j.aca.2007.03.055 |
| Abstrakt: |
Oxazepam is the major metabolite screened in urine samples for the evidence of the use of benzodiazepine drugs. The methods currently used, however, are laborious and time consuming. This paper proposes an oxazepam detection method based on its hydrolysis and cyclization--a reaction catalysed by cerium (IV) in an ortho-phosphoric acid-containing medium--to form 2-chloro-9(10H)-acridinone, a strongly fluorescent molecule. The variables involved in the hydrolysis and cyclization stages were optimised. Oxazepam was detectable in the 5-900 ng mL(-1) range, with a detection limit of 4.15 ng mL(-1) for k=3. The method was successfully used for the determination of oxazepam in urine samples collected at different times after the oral administration of Valium and Tranxilium. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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