| Autor: |
Djimde AA; Centre de recherche et de formation sur le paludisme, Département d'épidemiologie des affections parasitaires, Faculté de medecine, de pharmacie et d'odonto-stomatologie, Université de Bamako, Mali. adjimde@mrtcbko.org, Kirkman L, Kassambara L, Diallo M, Plowe CV, Wellems TE, Doumbo OK |
| Jazyk: |
francouzština |
| Zdroj: |
Bulletin de la Societe de pathologie exotique (1990) [Bull Soc Pathol Exot] 2007 Feb; Vol. 100 (1), pp. 3-5. |
| DOI: |
10.3185/pathexo2883 |
| Abstrakt: |
Malaria immunology, molecular biology and pathogenicity studies often require the adaptation of Plasmodium falciparum field isolates to continuous in vitro cultivation. For this purpose we have established propagation protocols of asexual erythrocytic stages of P. falciparum samples from malaria patients or asymptomatic carriers in Mali. The parasites were grown in standard culture medium supplemented by human serum and in a culture medium without human serum but supplemented by AlbuMax 1. The candle jar environment and tissue culture flasks gassed with 5% CO2, 5% O2 and 90% N2 obtained from a portable gas mixer were used. Protocols for parasite cultivation in a resource-poor setting were developed. These protocols were successfully applied to fresh isolates in Mali as well as to blood samples frozen in liquid nitrogen and shipped to a laboratory in U.S.A. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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