| Autor: |
Tsuruta T; Division of Pharmacy, Saga University Medical Hospital. tsuruta@cc.saga-u.ac.jp, Yang C, Ueki H, Li G, Maekawa A, Kamikawa H, Oku E, Somehara T, Fujito H, Tatebayashi H, Yamada S |
| Jazyk: |
angličtina |
| Zdroj: |
Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology [Nihon Shinkei Seishin Yakurigaku Zasshi] 2007 Feb; Vol. 27 (1), pp. 9-12. |
| Abstrakt: |
A rapid and sensitive high-performance liquid chromatographic method was validated and described for determination of paroxetine in human saliva. Following liquid-liquid extraction of the drug and an internal standard (dibucaine), chromatographic separation was accomplished using a C18 analytical column with a mobile phase consisting of 0.05 mol/L sodium phosphate buffer, pH 5.0, and acetonitrile (A 30:70, v/v; B 60:40, v/v). Paroxetine and the internal standard were detected by ultraviolet absorbance at 205 nm. The average recoveries of the drug and internal standard were 92.5% and 89%, respectively. The lower limits of detection and quantification were 1 and 4 ng/ml, respectively, and the calibration curve was linear over a concentration range of 4 ng/ml. The saliva level of paroxetine in patients with depression taking 10 to 40 mg/day of the drug was significantly correlated with the plasma level of paroxetine in each patient (r = 0.617, P < 0.004, n = 19). These data indicate that the saliva level of paroxetine could be a useful marker to predict the plasma level of the drug. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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