| Autor: |
Kanin EI; Department of Biochemistry and Genome Center of Wisconsin, University of Wisconsin, Madison, WI 53706, USA., Kipp RT, Kung C, Slattery M, Viale A, Hahn S, Shokat KM, Ansari AZ |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2007 Apr 03; Vol. 104 (14), pp. 5812-7. Date of Electronic Publication: 2007 Mar 28. |
| DOI: |
10.1073/pnas.0611505104 |
| Abstrakt: |
The process of gene transcription requires the recruitment of a hypophosphorylated form of RNA polymerase II (Pol II) to a gene promoter. The TFIIH-associated kinase Cdk7/Kin28 hyperphosphorylates the promoter-bound polymerase; this event is thought to play a crucial role in transcription initiation and promoter clearance. Studies using temperature-sensitive mutants of Kin28 have provided the most compelling evidence for an essential role of its kinase activity in global mRNA synthesis. In contrast, using a small molecule inhibitor that specifically inhibits Kin28 in vivo, we find that the kinase activity is not essential for global transcription. Unlike the temperature-sensitive alleles, the small-molecule inhibitor does not perturb protein-protein interactions nor does it provoke the disassociation of TFIIH from gene promoters. These results lead us to conclude that other functions of TFIIH, rather than the kinase activity, are critical for global gene transcription. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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