| Abstrakt: |
The influence of a 0.72 cGy/day dose rate of gamma-radiation on plasma membranes of peripheral blood lymphocytes of rats exposed to the doses of 1.5, 15, 30, 60 and 100 cGy was studied. Parameters characterizing the viscosity and the polarity of lipid bilayer and also an external membrane surface properties were examined using fluorescent probes pyrene and 1-anilinonaphthalene-8-sulfonate (ANS). Was shown the membrane structural parameters alterations after animal exposure to the doses of 1.5, 15, 60 and 100 cGy, being of a nonmonotonous nature as the dose accumulated. After exposure to the doses lower then than 30 cGy spectral changes were revealed not in each particular experiment that was probably caused by the individual peculiarities of radiation response development. After exposure to the doses higher than 30 cGy the changes were of reproducible character. After a 1.5 cGy dose a slight lipid bilayer polarity decrease and ANS binding parameter multidirectional changes were observed. After exposure to 15, 60 and to 100 cGy was shown polarity elevation and repartition of polar groups within the bilayer, the increase of viscosity of more polar membrane regions and also ANS fluorescence reduction mostly at the expense of quantum yield decrease. After the exposure of 60 cGy was observed a viscosity decrease in hydrophobic regions along with viscosity increase in more polar regions and after a 100 cGy dose accumulation an essential surface charge shift was found. Revealed alterations indicate the reorganization of external membrane surface and of intensification of oxidative processes in lipid bilayer. |
