Targeting neural circuitry in zebrafish using GAL4 enhancer trapping.

Autor: Scott EK; Department of Physiology, University of California, San Francisco, California 94158, USA., Mason L, Arrenberg AB, Ziv L, Gosse NJ, Xiao T, Chi NC, Asakawa K, Kawakami K, Baier H
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2007 Apr; Vol. 4 (4), pp. 323-6. Date of Electronic Publication: 2007 Mar 18.
DOI: 10.1038/nmeth1033
Abstrakt: We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
Databáze: MEDLINE