Acute respiratory infection by human metapneumovirus in children in southern Brazil.

Autor: do Carmo Debur M; Laboratório de Virologia, Hospital de Clínicas da Universidade Federal do Paraná, Brazil., Bordignon J, Duarte dos Santos CN, Vidal LR, Nogueira MB, de Almeida SM, Raboni SM
Jazyk: angličtina
Zdroj: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2007 May; Vol. 39 (1), pp. 59-62. Date of Electronic Publication: 2007 Mar 12.
DOI: 10.1016/j.jcv.2007.01.012
Abstrakt: Background: Human metapneumovirus (hMPV) has been described as an etiologic agent of acute respiratory infections (ARI), mainly in pediatric patients. Viral isolation is difficult and has low sensitivity, and consequently RT-PCR assays are currently used for detection.
Objectives: Detect hMPV in ARI in hospitalized children in Southern Brazil; standardize a RT-PCR for routine hMPV diagnosis; validate a positive control for molecular tests; and perform phylogenetics analyses.
Study Design: Nasopharyngeal aspirates (NPA) from 156 hospitalized children were studied. A conserved region of the nucleoprotein gene was cloned, characterized and used to standardize an RT-PCR assay. Phylogenetic analyses were performed. Clinical data were obtained from medical records.
Results: hMPV was detected in 6.4% of the samples. Dyspnea and wheezing were frequently reported symptoms and the most common diagnoses were bronchiolitis, acute respiratory insufficiency or laryngotracheobronchitis. Nucleotide sequence alignment revealed 97.7% identity with genotype A1 of hMPV. The detection limit of hMPV genomes by RT-PCR in clinical samples was 180 copies/microL.
Conclusion: This is the first report of the detection and genetic characterization of hMPV infections in children with lower ARI in Southern Brazil.
Databáze: MEDLINE
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