| Autor: |
Bolder SG; DMV International b.v., P.O. Box 13, 5460 BA Veghel, The Netherlands., Sagis LM, Venema P, van der Linden E |
| Jazyk: |
angličtina |
| Zdroj: |
Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2007 Apr 10; Vol. 23 (8), pp. 4144-7. Date of Electronic Publication: 2007 Mar 07. |
| DOI: |
10.1021/la063048k |
| Abstrakt: |
The conversion of protein monomers into fibrils can be determined using the centrifugal filtration method. The results of this method were used to calibrate steady-shear birefringence and Thioflavin T fluorescence measurements. For both measurements, a linear correlation with the fibril concentration was extracted, resulting in two fast assays to determine the fibril concentration quantitatively. From birefringence measurements and the conversion determined using the centrifugal filtration method, we were able to calculate more precise values for the birefringence per unit length of the fibrils (M) and the flexibility of the fibrils (beta). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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