| Autor: |
Thestrup-Pedersen K; Section of Dermatology, Department of Internal Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia. Ktp5@hotmail.com, Parhar R, Wu K, Bertilsson PA, Meyer B, Abu-Amero S, Hainau B, Aleisa A, Alfadley A, Hamadah I, Alajlan A, Al-Hussein K, Al-Mohanna F |
| Jazyk: |
angličtina |
| Zdroj: |
Acta dermato-venereologica [Acta Derm Venereol] 2007; Vol. 87 (2), pp. 118-26. |
| DOI: |
10.2340/00015555-0206 |
| Abstrakt: |
A total of 27 T-lymphocyte cell strains were established from skin biopsies of 24 patients with various stages of cutaneous T-cell lymphoma (CTCL) by addition of the T-cell growth factors interleukin (IL)-2 and IL-4. Cellular proliferation and phenotypic changes were measured over 3 months in culture, and T-cell clones were studied using T-cell receptor-? re-arrangement techniques. An average outgrowth of 134 million T-lymphocytes from a 4-mm skin biopsy was observed over 2 months. Initially, most T-cells expressed the CD4+ phenotype. In 17 cell strains from patients with early CTCL a statistically significant predominance of CD8+ T-lymphocytes developed over 8-weeks' culture, indicating that CD8+ T-cells controlled the growth of CD4+ T cells, whereas CD4+ T-cells were predominant in cell strains from advanced CTCL (p <0.05). TCR-? re-arrangement studies revealed, on average, 12 T-cell clones per cell strain, which was reduced over time to 6 T-cell clones per cell strain. Lymphocytes from peripheral blood could kill lymphocytes from an autologous cell strain, suggesting the presence of autoreactive cytotoxic T-cells. Our study suggests how skin-homing CD8+ T-lymphocytes from patients with early stage CTCL can suppress the in vitro growth of skin-homing CD4+ T-lymphocytes, indicating immune surveillance. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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