| Autor: |
Aalizadeh N; Div. of Immunology, Dept. of Pathobiology School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran. khorami@yahoo.com., Pezeshki M, Korramizadeh MR, Eslami MB, Zeraati H |
| Jazyk: |
angličtina |
| Zdroj: |
Iranian journal of allergy, asthma, and immunology [Iran J Allergy Asthma Immunol] 2003 Sep; Vol. 2 (3), pp. 159-63. |
| DOI: |
02.03/ijaai.159163 |
| Abstrakt: |
There are increasing data on novel tumor markers such as gelatinase A, which play a key role in tissue invasion and metastasis. Since prostate cancer is one of the common malignancies, we designed a simple and applicable Indirect Hemagglutination (IHA) test for determination of total gelatinase A in serum samples. In this study, we have analyzed the circulating form of gelatinase A (MMP-2) in patients suffering from either benign prostate hyperplasia (n= 54) or prostate cancer (n= 26) and normal individuals as control (n= 26). The gelatinolytic activity was determined by zymography followed by densitometric analysis. PSA was quantified by using a standard ELISA technique. Correlation of densitometric analysis of gelatinase A activity and IHA titer was significant at 0.01 level (p< 0.01, r = 0.916). Correlation of PSA and IHA titer was significant at 0.01 level (p< 0.01, r = 0.746). Border line IHA titer in patients with prostate cancer was 512 +/- 1 tube titer, in benign prostate hyperplasia patients was 128 +/- 1 tube titer, and the titer in normal individuals was 8 +/- 1 tube titer. These results demonstrate that IHA compared to zymography may be a better and simpler procedure in monitoring and screening patients with prostate cancer. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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