| Autor: |
Y Waye M; Department of Biochemistry, The Croucher Laboratory for Human Genomics, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China., W Law P, Wong CH, C Au T, Chuck CP, Kong SK, S Chan P, To KF, I Lo A, W Chan J, Suen YK, Edwin Chan HY, Fung KP, Y Sung J, Lo YM, W Tsui S |
| Jazyk: |
angličtina |
| Zdroj: |
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference [Conf Proc IEEE Eng Med Biol Soc] 2005; Vol. 2005, pp. 7482-5. |
| DOI: |
10.1109/IEMBS.2005.1616242 |
| Abstrakt: |
An outbreak of severe acute respiratory syndrome (SARS) occurred in China and the first case emerged in mid November 2002. The etiologic agent of this disease was found to be a previously unknown coronavirus, SARS-CoV. The detailed pathology of SARS-CoV infection and the host response to the viral infection are still not known. The 3a gene encodes a non-structural viral protein which is predicted to be a transmembrane protein. In this study, we showed that the 3a protein was localized to the endoplasmic reticulum (ER) in 3a-transfected monkey kidney Vero E6 cells. In vitro experiments of chromatin condensation and DNA fragmentation suggest that the 3a protein may trigger apoptosis. Our data show that over-expression of a single SARS-CoV protein can induce apoptosis in vitro. Thus GFP-3a fusion protein could also be used as a biosensor for monitoring the cytopathic features of SARS infection, e.g. lymphopenia, in animal model systems, similar to nucleocapsid and 7a proteins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
