| Autor: |
Zagorov MY; Department of Microbiology and Immunology, Medical University-Plovdiv, 15A Vassil Aprilov Street, 4000 Plovdiv, Bulgaria., Draganov MM, Alimanska SA, Staykova ND, Stefanov RS, Trayancheva M, Kuzmanova SI, Murdjeva MA |
| Jazyk: |
angličtina |
| Zdroj: |
Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2007 May; Vol. 30 (3), pp. 153-62. Date of Electronic Publication: 2007 Jan 22. |
| DOI: |
10.1016/j.cimid.2006.11.007 |
| Abstrakt: |
Indirect immunofluorescent assay for antinuclear antibodies (IIFA-ANA) on HEp-2 cell substrate is a widely used test for diagnosis of connective tissue diseases. Recently, serum-free fibroblast cell line McCoy-Plovdiv has been developed to provide an alternative substrate. The aim of the present study was to evaluate the diagnostic performance of IIFA-ANA on McCoy-Plovdiv cell substrate for systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and compare it with that of the standard HEp-2 cell substrate. Sera from 72 patients with RA and 23 patients with SLE were tested with IIFA-ANA on both substrates. The control group consisted of 100 sera from healthy individuals. The agreement (Cohen's kappa) and the diagnostic performance (ROC analysis) of both methods were evaluated. IIFA-ANA on McCoy-Plovdiv cells proved to be comparable with the standard IIFA-ANA on HEp-2 cell substrate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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