[Role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblast].

Autor:	Yang F; Experimental and Research Center, North China Coal Medical College, Tangshan 063000, China., Zhu XL, Wang LP, Song XD, Wang RM, Li ZG, Luo L, Hu WM, Ma WD, Pei X, Zhang LJ, Li QJ
Jazyk:	čínština
Zdroj:	Zhonghua xin xue guan bing za zhi [Zhonghua Xin Xue Guan Bing Za Zhi] 2006 Sep; Vol. 34 (9), pp. 843-6.
Abstrakt:	Objective: To investigate the role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblasts. Methods: Neonatal rat cardiac fibroblasts were isolated and stimulated by PDGF. The cell proliferation was observed by (3)H-TdR incorporation assay. The synthesis of collagen was measured by (3)H-proline incorporation assay. The expression of type I and type III collagen and MMP-1 protein were measured by Western blot. The MMP-2 and MMP-9 activity was evaluated with zymography assay. Results: PDGF stimulated cardiac fibroblasts proliferation with increased collagen synthesis and type I and type III collagen protein expressions as well as MMP-2 and MMP-9 activities and MMP-1 expression. AcSDKP inhibited cardiac fibroblasts proliferation induced by PDGF and reduced collagen synthesis and type I and type III collagen protein expression. AcSDKP also further up-regulated MMP-2 and MMP-9 activities and MMP-1 expression in cardiac fibroblasts induced by PDGF. Conclusion: AcSDKP inhibited proliferation and collagen synthesis and up-regulated matrix metalloproteinases activity or expression induced by PDGF, which was possibly related with the effect of AcSDKP anti-fibrosis.
Databáze:	MEDLINE
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