| Autor: |
Huisman SM; Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK., Smeets MF, Segal M |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of cell science [J Cell Sci] 2007 Feb 01; Vol. 120 (Pt 3), pp. 435-46. Date of Electronic Publication: 2007 Jan 09. |
| DOI: |
10.1242/jcs.03342 |
| Abstrakt: |
Spindle morphogenesis is regulated by cyclin-dependent kinases and monitored by checkpoint pathways to accurately coordinate chromosomal segregation with other events in the cell cycle. We have previously dissected the contribution of individual B-type cyclins to spindle morphogenesis in Saccharomyces cerevisiae. We showed that the S-phase cyclin Clb5p is required for coupling spindle assembly and orientation. Loss of Clb5p-dependent kinase abolishes intrinsic asymmetry between the spindle poles resulting in lethal translocation of the spindle into the bud with high penetrance in diploid cells. This phenotype was exploited in a screen for high dosage suppressors that yielded spc110(Delta)(13), encoding a truncation of the spindle pole body component Spc110p (the intranuclear receptor for the gamma-tubulin complex). We found that Clb5p-GFP was localised to the spindle poles and intranuclear microtubules and that Clb5p-dependent kinase promoted cell cycle dependent phosphorylation of Spc110p contributing to spindle integrity. Two cyclin-dependent kinase consensus sites were required for this phosphorylation and were critical for the activity of spc110(Delta)(13) as a suppressor. Together, our results point to the function of cyclin-dependent kinase phosphorylation of Spc110p and provide, in addition, support to a model for Clb5p control of spindle polarity at the level of astral microtubule organisation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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