| Autor: |
Laudencia-Chingcuanco DL; Genomics and Gene Discovery, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710, USA. dlc@pw.usda.gov, Stamova BS, You FM, Lazo GR, Beckles DM, Anderson OD |
| Jazyk: |
angličtina |
| Zdroj: |
Plant molecular biology [Plant Mol Biol] 2007 Mar; Vol. 63 (5), pp. 651-68. Date of Electronic Publication: 2007 Jan 09. |
| DOI: |
10.1007/s11103-006-9114-y |
| Abstrakt: |
The expression of 7,835 genes in developing wheat caryopses was analyzed using cDNA arrays. Using a mixed model analysis of variance (ANOVA) method, 29% (2,237) of the genes on the array were identified to be differentially expressed at the 6 different time-points examined, which covers the developmental stages from coenocytic endosperm to physiological maturity. Comparison of genes differentially expressed between two time-points revealed a dynamic transcript accumulation profile with major re-programming events that occur at 3-7, 7-14 and 21-28 DPA. A k-means clustering algorithm grouped the differentially expressed genes into 10 clusters, revealing co-expression of genes involved in the same pathway such as carbohydrate and protein synthesis or preparation for desiccation. Functional annotation of genes that show peak expression at specific time-points correlated with the developmental events associated with the respective stages. Results provide information on the temporal expression during caryopsis development for a significant number of differentially expressed genes with unknown function. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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