Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots.

Autor: Innocenti G; Interactions Plantes-Microorganismes et Santé Végétale, UMR INRA 1064/Université de Nice-Sophia Antipolis/CNRS 6192, 400, route des Chappes, BP 167, 06903, Sophia-Antipolis, France., Pucciariello C, Le Gleuher M, Hopkins J, de Stefano M, Delledonne M, Puppo A, Baudouin E, Frendo P
Jazyk: angličtina
Zdroj: Planta [Planta] 2007 May; Vol. 225 (6), pp. 1597-602. Date of Electronic Publication: 2006 Dec 30.
DOI: 10.1007/s00425-006-0461-3
Abstrakt: Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (gamma-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that gamma-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.
Databáze: MEDLINE