Deduced amino acid sequences surrounding the fusion glycoprotein cleavage site and of the carboxyl-terminus of haemagglutinin-neuraminidase protein of the avirulent thermostable vaccine strain I-2 of Newcastle disease virus.

Autor: Wambura PN; School of Veterinary Science, University of Queensland, Brisbane, Australia. phil_Wambura@yahoo.com, Meers J, Kattenbelt JA, Gould AR, Spradbrow PB
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2007 Jan; Vol. 31 (1), pp. 105-12. Date of Electronic Publication: 2006 Dec 29.
DOI: 10.1007/s11259-006-3290-8
Abstrakt: A single-tube RT-PCR technique generated a 387 bp or 300 bp cDNA amplicon covering the F(0) cleavage site or the carboxyl (C)-terminus of the HN gene, respectively, of Newcastle disease virus (NDV) strain I-2. Sequence analysis was used to deduce the amino acid sequences of the cleavage site of F protein and the C-terminus of HN protein, which were then compared with sequences for other NDV strains. The cleavage site of NDV strain I-2 had a sequence motif of (112) RKQGRLIG(119), consistent with an avirulent phenotype. Nucleotide sequencing and deduction of amino acids at the C-terminus of HN revealed that strain I-2 had a 7-amino-acid extension (VEILKDGVREARSSR. This differs from the virulent viruses that caused outbreaks of Newcastle disease in Australia in the 1930s and 1990s, which have HN extensions of 0 and 9 amino acids, respectively. Amino acid sequence analyses of the F and HN genes of strain I-2 confirmed its avirulent nature and its Australian origin.
Databáze: MEDLINE
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