| Autor: |
Pincus DW; Department of Neurology, Cornell University Medical College, New York, NY 10021., DiCicco-Bloom E, Black IB |
| Jazyk: |
angličtina |
| Zdroj: |
Brain research [Brain Res] 1991 Jul 12; Vol. 553 (2), pp. 211-4. |
| DOI: |
10.1016/0006-8993(91)90827-i |
| Abstrakt: |
The present study examines the role of Ca2+ in the regulation of sympathetic neuroblast mitosis. Employing a fully defined neuroblast culture system, we previously found that insulin growth factors (IGFs), depolarization and vasoactive intestinal peptide (VIP) regulated precursor mitosis. We now report that Ca2+ entry via voltage-sensitive channels was required for depolarization-stimulated mitogenesis. Ca2+ channel blockade with nitrendipine completely inhibited the increase in [3H]thymidine incorporation elicited by depolarizing stimuli including 30 mM KCl and the Na+ channel agonist veratridine. However, Ca2+ channel activity was not involved in the stimulation of DNA synthesis by IGFs or VIP. Thus, neuroblast mitosis may be regulated by multiple intracellular as well as extracellular signals. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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