| Autor: |
Serebrianyĭ VA, Sinitsyna OA, Fedorova EA, Okunev ON, Bekkarevich AO, Sokolova LM, Vinetskiĭ IuP, Sinitsyn AP |
| Jazyk: |
ruština |
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2006 Nov-Dec; Vol. 42 (6), pp. 665-73. |
| Abstrakt: |
A fragment of Penicillium canescens genomic DNA carrying the xlnR gene coding for a translational activator of xylanolytic genes was isolated. It was demonstrated that a loss of this function in genetically modified transformants resulted in a drastic decrease in the production of P. canescens major xylanases and had a negative effect on the syntheses of several other extracellular xylanases. An increase in the dose of the xlnR gene elevated the xylanolytic activity as well as the activities of a number of other auxiliary enzymes involved in xylan degradation. The activities of two P. canescens major secreted enzymes--beta-galactosidase and alpha-L-arabinofuranosidase-appeared weakly dependent on the translational activator xlnR. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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