C8orf4/TC-1 (thyroid cancer-1) gene expression in thyroid cancer and goiter.
| Autor: | de Melo Martins PC; Laboratory of Molecular Genetics, São Paulo, Brazil., Parise Junior O, Pereira Hors C, Villela Miguel RE, da Costa Andrade VC, Garicochea B |
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| Jazyk: | angličtina |
| Zdroj: | ORL; journal for oto-rhino-laryngology and its related specialties [ORL J Otorhinolaryngol Relat Spec] 2007; Vol. 69 (2), pp. 127-30. Date of Electronic Publication: 2006 Dec 13. |
| DOI: | 10.1159/000097980 |
| Abstrakt: | Background: The expression of the thyroid cancer-1(TC-1) gene seems to be related with malignant transformation in the thyroid tissue. Objective: We evaluated the potential use of TC-1 gene expression as a marker of malignancy in thyroid nodules. Methods: A total of 92 frozen thyroid samples were studied, including 46 samples from thyroid nodules (19 papillary carcinomas, 1 follicular carcinoma, 24 adenomatous goiters, and 2 follicular adenomas) and 46 samples from normal surrounding thyroid tissue. Total RNA was extracted and TC-1 expression was assessed by semiquantitative Multiplex PCR. Results were verified using real-time RT-PCR in some of the samples. Results: Overall mean TC-1 gene expression (normalized by the ABL gene) was 1.73 +/- 1.67 (0.33-9.33). There was a significant difference (p < 0.001) between TC-1 gene expression in benign thyroid lesions (1.07 +/- 0.10) and carcinomas (2.73 +/- 0.51). Conclusion: Our results suggest that TC-1 gene expression may be useful in the differential diagnosis of goiters and thyroid papillary carcinomas. |
| Databáze: | MEDLINE |
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