| Autor: |
Pereira de Jesus D; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil., Blanes L, do Lago CL |
| Jazyk: |
angličtina |
| Zdroj: |
Electrophoresis [Electrophoresis] 2006 Dec; Vol. 27 (24), pp. 4935-42. |
| DOI: |
10.1002/elps.200600137 |
| Abstrakt: |
In this work, a microfluidic free-flow electrophoresis device, obtained by thermal toner transferring on glass substrate, is presented. A microdevice can be manufactured in only 1 h. The layout of the microdevice was designed in order to improve the fluidic and electrical characteristics. The separation channel is 8 microm deep and presents an internal volume of 1.42 microL. The deleterious electrolysis effects were overcome by using a system that isolates the electrolysis products from the separation channel. The Joule heating dissipation in the separation channel was found to be very efficient up to a current density of 8.83 mA/mm(2) that corresponds to a power dissipation per unit volume of running electrolyte of 172 mW/microL. Promising results were obtained in the evaluation of the microdevices for the separation of ionic dyes. The microfluidic device can be used for a continuous sample pretreatment step for micro total analysis system. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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