[An immunoenzyme method of detecting Vibrio cholerae cytolysin].

Autor: Babitsyn SN, Tsittser AO, Semiotrochev VL, Sedina SG
Jazyk: ruština
Zdroj: Laboratornoe delo [Lab Delo] 1991 (5), pp. 61-2.
Abstrakt: Enzyme immunoassay was used in detection of V. cholerae cytolysin. Conjugate of immunoglobulins to purified cytolysin with horseradish peroxidase was used, obtained by the periodate technique. The method sensitivity is 2 ng/ml of purified cytolysin. The results of hemolytic activity measurements in supernatants of 40 V. cholerae strains and enzyme immunoassay findings were in high correlation.
Databáze: MEDLINE