| Autor: |
Burbelo PD; Department of Oncology, Georgetown University Medical Center, Washington, DC 20057, USA. burbp1@yahoo.com, Ching KH, Mattson TL, Light JS, Bishop LR, Kovacs JA |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2007 Jan 26; Vol. 352 (4), pp. 889-95. Date of Electronic Publication: 2006 Dec 06. |
| DOI: |
10.1016/j.bbrc.2006.11.140 |
| Abstrakt: |
The application of LIPS to the rapid quantification of antibody responses to infectious agents is described. Chimeric genes encoding pathogen antigens fused to Renilla luciferase are expressed in mammalian cells; crude extracts are prepared and, without purification, employed in immunoprecipitation assays to quantify pathogen-specific antibodies. In cross-sectional and longitudinal studies, antibody levels to the MSG-14 antigen of Pneumocystis jirovecii measured by this assay correlated well with levels previously obtained with an optimized ELISA. We also correctly predicted Hepatitis B (HBV), Hepatitis C (HCV), and HIV infection status in all but 2 of 99 assays analyzing 33 patient sera. We then used 15 HIV-encoded proteins comprising the whole HIV proteome to generate antibody response profiles for these 33 sera. Each HIV antigen was recognized by antibodies in serum from at least one HIV-infected individual. Data generated with these simple, quantitative antibody-detection assays have both clinical and research applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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