| Autor: |
Mack DO; H. L. Snyder Memorial Research Foundation, Winfield, KS., Smith LD |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry international [Biochem Int] 1991 Mar; Vol. 23 (5), pp. 855-60. |
| Abstrakt: |
Adenylosuccinase catalyses the conversion of adenylosuccinic acid to AMP and fumarate. We have developed a coupled enzyme staining procedure applicable to nitrocellulose blots after agarose gel isoelectrofocusing of rat muscle adenylosuccinase. The coupling enzymes, fumarase (fumarate to L-malate) and malic enzyme (L-malate to pyruvate and NADPH), are adsorbed to nitrocellulose prior to blotting. The NADPH, mediated by phenazine methosulfate, converts a tetrazolium salt to its blue formazan. This procedure demonstrated that rat muscle adenylosuccinase consists of three isomeric forms present in similar amounts. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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