| Autor: |
Eller CD; UCLA Department of Human Genetics, David Geffen School of Medicine, Gonda Center, 695 E. Young Drive South, Los Angeles, California 90095-7088, USA., Regelson M, Merriman B, Nelson S, Horvath S, Marahrens Y |
| Jazyk: |
angličtina |
| Zdroj: |
Gene [Gene] 2007 Apr 01; Vol. 390 (1-2), pp. 153-65. Date of Electronic Publication: 2006 Oct 05. |
| DOI: |
10.1016/j.gene.2006.09.018 |
| Abstrakt: |
Housekeeping genes are expressed across a wide variety of tissues. Since repetitive sequences have been reported to influence the expression of individual genes, we employed a novel approach to determine whether housekeeping genes can be distinguished from tissue-specific genes by their repetitive sequence context. We show that Alu elements are more highly concentrated around housekeeping genes while various longer (>400-bp) repetitive sequences ("repeats"), including Long Interspersed Nuclear Element-1 (LINE-1) elements, are excluded from these regions. We further show that isochore membership does not distinguish housekeeping genes from tissue-specific genes and that repetitive sequence environment distinguishes housekeeping genes from tissue-specific genes in every isochore. The distinct repetitive sequence environment, in combination with other previously published sequence properties of housekeeping genes, was used to develop a method of predicting housekeeping genes on the basis of DNA sequence alone. Using expression across tissue types as a measure of success, we demonstrate that repetitive sequence environment is by far the most important sequence feature identified to date for distinguishing housekeeping genes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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