| Autor: |
Kruse C; Fraunhofer-lnstitute of Biomedical Engineering, Group of Cell Differentiation and Cell Technology at the University of Lübeck, MFC Innovationscampus 1, Maria-Goeppert-Strasse 1, D-23538 Lübeck, Germany. charli.kruse@ibmt.fhg.de, Kajahn J, Petschnik AE, Maass A, Klink E, Rapoport DH, Wedel T |
| Jazyk: |
angličtina |
| Zdroj: |
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft [Ann Anat] 2006 Nov; Vol. 188 (6), pp. 503-17. |
| DOI: |
10.1016/j.aanat.2006.07.012 |
| Abstrakt: |
Cells isolated from pancreas have a remarkable potential for self-renewal and multilineage differentiation. We here present a comprehensive characterisation of stem/progenitor cells derived from exocrine parts of the adult rat pancreas. Using purified cells from either single colonies or even single-cell clones, we specifically demonstrate: (i) the cells contain the typical stem/progenitor cell markers alkaline phophatase, SSEA-1, Oct-4, CD9, Nestin, Pax6, CD44, a-Fetoprotein and Brachyury, demonstrated by immunocytochemistry and RT-PCR; (ii) the cells have the potential to differentiate into lineages of all three germ layers in vitro; (iii) a clonal analysis revealed that even cell lines derived from a single cell have stem/progenitor cell properties such as self-renewal and spontaneous differentiation into various cell lineages; (iv) the cells have the propensity to form three-dimensional, teratoma-like structures in vitro, which contain cells of different lineages; and (v) external stimuli can activate the generation of certain cell types. For instance, cells treated with retinoic acid show an increased expression of alpha-smooth muscle actin. These results suggest that exocrine glands, such as pancreas may be a potential source of adult stem/progenitor cells, suitable for cell therapy of degenerative diseases. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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