| Autor: |
Tatarinov YS; Department of Biochemistry and Immunochemical, Second Moscow Medical Institute, USSR., Terentiev AA, Moldogazieva NT, Tagirova AK |
| Jazyk: |
angličtina |
| Zdroj: |
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 1991; Vol. 12 (3), pp. 125-30. |
| DOI: |
10.1159/000217697 |
| Abstrakt: |
Human alpha-fetoprotein (AFP) was isolated from human abortive tissue by biospecific chromatography on immobilized estrogens. The most effective sorbents were: estrone-0-3-hemisuccinyl-hexamethylenediamine-Sepharose CL 4B and diethylstilbestrol-diasoanisole-sulfonyl-oxyethyl-Sepharose CL 4B. As elution solution the most optimum was 10% buffered aqueous butanol. Taking into consideration the data obtained, one can conclude that AFP in human biological fluids is bound to immobilized estrogens. Butanol extraction deestrogenizes AFP, and as a result human AFP acquires affinity to immobilized estrogens. During rechromatography on immobilized diethylstilbestrol, it was possible to obtain AFP preparations of about 95% purity. The present results provide the opportunity to work out new methodological approaches to human AFP isolation using biospecific chromatography on immobilized estrogens. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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