A novel methodology for imaging new bone formation around bioceramic bone substitutes.

Autor: Gorustovich AA; Research Laboratory, National Atomic Energy Commission (CNEA-Regional Noroeste), A4408FTV Salta, Argentina. alegorustov@ciudad.com.ar, Sivak MG, Guglielmotti MB
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2007 May; Vol. 81 (2), pp. 443-5.
DOI: 10.1002/jbm.a.31071
Abstrakt: In this study, Frost's bulk-staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol-soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.
(Copyright 2006 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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