The study of peripheral blood mononuclear cell MHC I and MHC II gene mRNA expression in acute graft rejection.

Autor: Li XD; Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, China., Zou X, Zhang Y, Lu N, Wan FR, Zhao SM, Li XL, Jiang H
Jazyk: angličtina
Zdroj: Transplantation proceedings [Transplant Proc] 2006 Nov; Vol. 38 (9), pp. 3055-7.
DOI: 10.1016/j.transproceed.2006.08.185
Abstrakt: Background: Early diagnosis of acute graft rejection is important in the clinic. To explore a reliable diagnostic marker, we selected skin-grafted rabbits as an animal model to study peripheral blood mononuclear cell (PBMC) major histocompatibility complex 1 (MHC I) and MHC II gene mRNA in acute graft rejection (AGR).
Methods: Fifteen New Zealand white rabbits were randomly divided into three groups to observe skin graft rejection: three rabbits were in the autograft control group; six rabbits in a cyclosporine (CsA) treated allografted group; and the other six rabbits in untreated allografted group. The CsA-treated allografted group was given CsA (5 mg/kg) daily intramuscularly. PBMC samples were obtained every 2 days to detect by real-time polymerase chain reaction, PBMC MHC I and MHC II gene mRNA.
Results: MHC I and MHC II gene mRNA levels did not show any obvious change in the autografted controls. MHC I gene mRNA levels showed a slow increase in the CsA-treated allografted group, but no obvious change in the untreated allografted group. MHC II gene mRNA reached the highest level at 2 to 3 days before graft rejection appeared macroscopically in the CsA-treated allografted group and untreated allografted group, then decreasing to a low level.
Conclusion: Compared with MHC I gene mRNA expression, PBMC MHC II gene mRNA expression may be considered to be an earlier marker for AGR.
Databáze: MEDLINE
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