| Autor: |
Toonen RF; Department of Functional Genomics, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands., Wierda K, Sons MS, de Wit H, Cornelisse LN, Brussaard A, Plomp JJ, Verhage M |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2006 Nov 28; Vol. 103 (48), pp. 18332-7. Date of Electronic Publication: 2006 Nov 16. |
| DOI: |
10.1073/pnas.0608507103 |
| Abstrakt: |
Prompt recovery after intense activity is an essential feature of most mammalian synapses. Here we show that synapses with reduced expression of the presynaptic gene munc18-1 suffer from increased depression during intense stimulation at glutamatergic, GABAergic, and neuromuscular synapses. Conversely, munc18-1 overexpression makes these synapses recover faster. Concomitant changes in the readily releasable vesicle pool and its refill kinetics were found. The number of vesicles docked at the active zone and the total number of vesicles per terminal correlated with both munc18-1 expression levels and the size of the releasable vesicle pool. These data show that varying expression of a single gene controls synaptic recovery by modulating the number of docked, release-ready vesicles and thereby replenishment of the secretion capacity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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