Protective effect of resveratrol against oxidative damage of UVA irradiated HaCaT cells.
| Autor: | Chen ML; Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410078, China., Li J, Xiao WR, Sun L, Tang H, Wang L, Wu LY, Chen X, Xie HF |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2006 Oct; Vol. 31 (5), pp. 635-9. |
| Abstrakt: | Objective: To observe the photoprotective effect and possible mechanisms of resveratrol for ultraviolet A (UVA) irradiated HaCaT cells. Methods: HaCaT cells under UVA irradiation with 5J/cm(2) were interfered with 0.01 mmol/L and 0.1 mmol/L resveratrol. The testing objects were divided into a control and a UVA irradiation group, and then we detected the proliferation capacity with methylthiazdyl tetrazolium (MTT) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, content of maleic dialdehyde (MDA) with hydroxylamine, colorimetric, thiobarbituric acid (TBA) methods. The ultrastructure was observed under electron microscope. Results: Resveratrol could enhance the proliferation activity, SOD, GSH-Px activity of HaCaT cells under UVA irradiation, decrease the content of MDA in dose-dependent manner (P<0.05). The electron microscope revealed that resveratrol could relieve the injury of HaCaT cells' ultrastructure. Conclusion: Resveratrol can relieve the inhibition to HaCaT cell proliferation,injury of their ultrastructure and oxidation by UVA irradiation. The protection is dose-dependent. That resveratrol raises the oxidase activity and clears the oxyradical may account for these results. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|