| Autor: |
Rivals JP; Institut de Biotechnologie, Bâtiment de Biologie, University of Lausanne, CH-1015 Lausanne, Switzerland., Plattet P, Currat-Zweifel C, Zurbriggen A, Wittek R |
| Jazyk: |
angličtina |
| Zdroj: |
Virology [Virology] 2007 Mar 01; Vol. 359 (1), pp. 6-18. Date of Electronic Publication: 2006 Oct 12. |
| DOI: |
10.1016/j.virol.2006.07.054 |
| Abstrakt: |
The wild-type canine distemper virus (CDV) strain A75/17 induces a non-cytocidal infection in cultures of canine footpad keratinocytes (CFKs) but produces very little progeny virus. After only three passages in CFKs, the virus produced 100-fold more progeny and induced a limited cytopathic effect. Sequence analysis of the CFK-adapted virus revealed only three amino acid differences, of which one was located in each the P/V/C, M and H proteins. In order to assess which amino acid changes were responsible for the increase of infectious virus production and altered phenotype of infection, we generated a series of recombinant viruses. Their analysis showed that the altered P/V/C proteins were responsible for the higher levels of virus progeny formation and that the amino acid change in the cytoplasmic tail of the H protein was the major determinant of cytopathogenicity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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