| Autor: |
Ivanov AP; Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20852, USA. ivanov@cber.fda.gov, Dragunsky EM, Ivanova OE, Rezapkin GV, Potapova SG, Chumakov KM |
| Jazyk: |
angličtina |
| Zdroj: |
Human vaccines [Hum Vaccin] 2005 May-Jun; Vol. 1 (3), pp. 102-5. Date of Electronic Publication: 2005 May 05. |
| DOI: |
10.4161/hv.1.3.1719 |
| Abstrakt: |
This study describes an ELISA variant (Binding Inhibition ELISA, BI ELISA) for the quantitative determination of neutralization-relevant antibodies to polioviruses. The test differs from previously described analogs by utilizing polyclonal immune reagents: capture antibodies and biotin-labeled detecting antibodies. The BI ELISA was compared with the conventional neutralization test (NT) by testing live Sabin and wild-type poliovirus strains. The comparison of 68 serum samples taken from Oral Poliovirus Vaccine (OPV) recipients showed 100% specificity and sensitivity for Sabin 1 and Sabin 2, and 100% sensitivity and 97% specificity for Sabin 3. Good correlations (r = 0.7, 0.77, 0.65 for Sabin 1, 2, 3, respectively) were demonstrated between the NT and BI ELISA results. These results indicate that the BI ELISA can be used as a reliable alternative to the NT for determination of neutralization-relevant antibodies to polioviruses in vaccinees and in large-scale sero-epidemiological studies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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