Presence of LDL modified by myeloperoxidase in the penis in patients with vascular erectile dysfunction: a preliminary study.

Autor: Zouaoui Boudjeltia K; Experimental Medicine Laboratory, ULB 222 Unit, ISPPC, CHU de Charleroi, Hôpital A. Vésale, Montigny-Le-Tilleul, Belgium., Roumeguere T, Delree P, Moguilevsky N, Ducobu J, Vanhaeverbeek M, Wespes E
Jazyk: angličtina
Zdroj: European urology [Eur Urol] 2007 Jan; Vol. 51 (1), pp. 262-8; discussion 268-9. Date of Electronic Publication: 2006 Sep 08.
DOI: 10.1016/j.eururo.2006.08.040
Abstrakt: Objective: Erectile dysfunction (ED) is a major vascular disorder. Atherosclerosis is closely related to lipoprotein metabolism and especially, oxidative modifications of low-density lipoproteins (LDLs), which are involved in early development of the atherosclerotic lesions. Current major questions include how LDLs are oxidised (OxLDL) in vivo. Myeloperoxidase (MPO) is an enzyme present in the azurophile granules of neutrophils and monocytes that can contribute to LDL oxidation in the presence of H(2)O(2). We have developed a new monoclonal antibody against LDL modified by MPO (Mox-LDL) and have used it on penile biopsies from patients operated on for penile implant.
Methods: Seven patients with vascular ED and one impotent patient after radical prostatectomy (RP) underwent biopsy of the cavernous body during penile implant procedures. An immunohistochemical study with a monoclonal antibody against Mox-LDL and an antibody against apoprotein B (ApoB), the protein moiety of LDL, to confirm the presence of LDL was performed.
Results: The staining was positive for Mox-LDL and ApoB and was present between the endothelial cells of the sinusoid spaces and the smooth muscle cells in the seven patients with vascular ED. The patient with RP was negative for Mox-LDL.
Discussion: Because it is known that modified LDL could decrease nitric oxide production, Mox-LDL could be one of the agents responsible for ED. Further studies are needed to confirm this hypothesis.
Databáze: MEDLINE
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