Characterization of bovine seminal plasma by proteomics.

Autor: Kelly VC; School of Biological Sciences, University of Auckland, Auckland, New Zealand., Kuy S, Palmer DJ, Xu Z, Davis SR, Cooper GJ
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2006 Nov; Vol. 6 (21), pp. 5826-33.
DOI: 10.1002/pmic.200500830
Abstrakt: Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species.
Databáze: MEDLINE