| Autor: |
Poulsen AK; CelCom, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark. allanpoulsen@bmb.sdu.dk, Petersen MØ, Olsen LF |
| Jazyk: |
angličtina |
| Zdroj: |
Biophysical chemistry [Biophys Chem] 2007 Feb; Vol. 125 (2-3), pp. 275-80. Date of Electronic Publication: 2006 Sep 01. |
| DOI: |
10.1016/j.bpc.2006.08.009 |
| Abstrakt: |
The observation of oscillations in the concentrations of NADH and other intermediates in glycolysis in dense yeast cell suspensions is generally believed to be the result of synchronization of such oscillations between individual cells. The synchrony is believed to be a property of cell density and the question is: does metabolism in each individual yeast cell continue to oscillate, but out of phase, in the absence of synchronization? Here we have used high-sensitivity fluorescence microscopy to measure NADH in single isolated yeast cells under conditions where we observe oscillations of glycolysis in dense cell suspensions. However, we have not been able to detect intracellular oscillations in NADH in these isolated cells, which cannot synchronize their metabolism with other cells. However, addition of acetaldehyde to a single cell as pulses with a frequency similar to the oscillations in dense cell suspensions will induce oscillations in that cell. Ethanol, another product of glycolysis, which has been proposed as a synchronizing agent of glycolysis in cells, was not able to induce oscillations when added as pulses. The experiments support the notion that the intracellular oscillations are associated with the cell density of the yeast cell suspension and mediated by acetaldehyde and perhaps also other substances. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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