| Autor: |
Piva E; Department of Operative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil. piva@ufpel.edu.br, Tarquínio SB, Demarco FF, Silva AF, de Araújo VC |
| Jazyk: |
angličtina |
| Zdroj: |
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics [Oral Surg Oral Med Oral Pathol Oral Radiol Endod] 2006 Oct; Vol. 102 (4), pp. e66-71. Date of Electronic Publication: 2006 Aug 04. |
| DOI: |
10.1016/j.tripleo.2006.01.015 |
| Abstrakt: |
The aim of this study was to investigate the expression of 2 extracellullar matrix glycoproteins, fibronectin (FNC) and tenascin (TNC), following direct pulp capping with calcium hydroxide (CH). Third molars scheduled for extraction were used. Standardized class I cavities with pulp exposures were prepared. After control of bleeding, CH powder was applied in the exposure sites, which were covered with CH cement (Dycal; Dentsply) and the cavities were filled with zinc oxide-eugenol cement. Three teeth were extracted at each post-treatment period (1, 7, 14, and 30 days). Demineralized and paraffin-embedded specimens were stained for histologic technique (hematoxylin-eosin) and for immunohistochemical analysis. Anti-TNC and anti-FNC monoclonal antibodies were used with the streptavidin-biotin complex method. Generally, similar patterns of immunohistochemical expression were observed for TNC and FNC in the pulp tissue as a whole. In the exposure site, TNC immunostaining increased over time, exhibiting a thicker immunostaining pattern within 30 days. The imunohistochemical technique showed expression of both glycoproteins during pulp healing process. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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