| Autor: |
Gaĭdamovich SIa, Loktev VB, Lavrova NA, Maksiutov AZ, Mel'nikova EE, Pereboev AV, Protopopov EV, Razumov IA, Sveshnikova NA, Khusainova AD |
| Jazyk: |
ruština |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 1990 May-Jun; Vol. 35 (3), pp. 221-5. |
| Abstrakt: |
Employment of radioimmunoassay led to the demonstration of serological crossing between tick-borne encephalitis (TBE) virus and Venezuelan equine encephalomyelitis (VEE) virus. Using hybridoma technology, three hybridomas were produced secreting monoclonal antibodies (MAb) cross-reacting with these two viruses. With MAb, the epitope of binding of these antibodies was shown to be located on protein E of TBE virus and protein E1 of VEE virus. Despite the low percentage (14%) of homology of amino acid sequences of these proteins, 12 areas with homology from 24% to 63% were demonstrated. Considering conservative replacements, homology of these areas was 53%-75%. The assumed existence of some of these areas in alpha-helical conformation may explain the observed immunological crossing. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
