| Autor: |
Sheludko YV; Institute of Cell Biology and Genetic Engineering, Zabolotnogo Str. 148, Kyiv 03143, Ukraine. ysheludko@fromru.com, Sindarovska YR, Gerasymenko IM, Bannikova MA, Kuchuk NV |
| Jazyk: |
angličtina |
| Zdroj: |
Biotechnology and bioengineering [Biotechnol Bioeng] 2007 Feb 15; Vol. 96 (3), pp. 608-14. |
| DOI: |
10.1002/bit.21075 |
| Abstrakt: |
Agrobacterium-mediated transient expression may be regarded as a promising method for inexpensive large-scale production of recombinant proteins. We optimized the protocol of transient expression in Nicotiana benthamiana and compared six Australian species of Nicotiana as hosts for transient expression. The transient expression of GFP under 35S CaMV promoter was observed in all species tested, although the GFP content in leaves of N. benthamiana, N. exigua, and N. excelsior was significantly higher (3.8, 3.7, and 2.0% TSP, respectively). Usage of viral-based expression system resulted in considerable increase of GFP accumulation in N. excelsior and N. benthamiana (63.5 and 16.2% TSP, respectively). We displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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