| Autor: |
Natochin M; Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA., Barren B, Ahmad ST, O'Tousa JE, Artemyev NO |
| Jazyk: |
angličtina |
| Zdroj: |
Vision research [Vision Res] 2006 Dec; Vol. 46 (27), pp. 4575-81. Date of Electronic Publication: 2006 Sep 18. |
| DOI: |
10.1016/j.visres.2006.07.013 |
| Abstrakt: |
Invertebrate and vertebrate rhodopsins share a low degree of homology and are coupled to G-proteins from different families. Here we explore the utility of fly-expressed chimeras between Drosophila rhodopsin Rh1 and bovine rhodopsin (Rho) to probe the interactions between the invertebrate and vertebrate visual pigments and their cognate G-proteins. Chimeric Rh1 pigments carrying individual substitutions of the cytoplasmic loops C2 and C3 and the C-terminus with the corresponding regions of Rho retained the ability to stimulate phototranduction in Drosophila, but failed to activate transducin. Surprisingly, chimeric Rho containing the Rh1 C-terminus was fully capable of transducin activation, indicating that the C-terminal domain of vertebrate rhodopsins is not essential for the functional coupling to transducin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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