| Autor: |
Oostdijk JP; Varian B.V., Department R&D Consumable Products, Herculesweg 8, 4338 PL Middelburg, The Netherlands. john.oostdijk@varianinc.com, Degenhardt CE, Trap HC, Langenberg JP |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. A [J Chromatogr A] 2007 May 25; Vol. 1150 (1-2), pp. 62-9. Date of Electronic Publication: 2006 Sep 11. |
| DOI: |
10.1016/j.chroma.2006.08.053 |
| Abstrakt: |
An improved method is presented for the trace analysis of sulfur mustard (HD) in biological samples, such as blood and tissue from laboratory animals. Using the internal standard method and liquid-liquid extraction with ethyl acetate, up to 400 microL of the extract was injected by thermal desorption from Tenax and analyzed by two-dimensional GC-MS/EI in SIM mode. The analysis was compared with a direct GC injection. Reversed thermal desorption was used as a tool for handling heavily contaminated (fat) samples, thus preventing contamination of the injection system and pre-column. A successful analytical configuration has been set up for the bioanalysis of HD at the low, toxicologically relevant pM level. A detection limit of 10 pg mL(-1) blood or pg g(-1) tissue of sulfur mustard (S/N=3) was established by using this configuration. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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