| Autor: |
Zhu GM; College of Animal Science and Technology, Northwest A&F University, Yangling, China., Chen H, Lu JS, Zhou YR, Wu XJ, Chen HX, Deng JX |
| Jazyk: |
čínština |
| Zdroj: |
Yi chuan = Hereditas [Yi Chuan] 2006 Sep; Vol. 28 (9), pp. 1123-8. |
| DOI: |
10.1360/yc-006-1123 |
| Abstrakt: |
Gene synthesis is very important in life science research, and it becomes a technique in common use. It is difficult for long gene synthesis, because the mismatches and mutations of DNA sequence in nucleotide fragments assembling. This study established a new method for long gene synthesis, which was referred to as PCR-restrict enzyme ligation method. With this method, a omega-3 fatty acid desaturase gene, sFat-1, from Caenorhabditis briggssae, was successfully assembled from 27 synthesized nucleotide fragments (60 ~ 68 bp for each fragment ) following 3 rounds of PCR (7 reactions) and 2 rounds of restrict enzyme ligation (3 reactions). This shows that the PCR-restrict enzyme ligation method is an effective method for long gene synthesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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